饲料中大豆异黄酮和大豆皂甙对牙鲆肝脏和肠道蛋白质消化酶活性和基因表达的影响     

米海峰  徐玮  麦康森  张文兵  陈伟  吴成龙 《中国海洋大学学报(自然科学版)》2011,41(12):40-45

以鱼粉为蛋白源,小麦粉为糖源,鱼油为脂肪源配制基础饲料,分别添加不同浓度梯度(0.1%、0.4%和0.8%)的大豆异黄酮和大豆皂甙。以不添加大豆异黄酮和大豆皂甙的基础饲料作为对照组,共制成7种等氮(粗蛋白49.1%)、等能(总能20.1kJ/g)的实验饲料。在循环水系统中养殖初始体质量为(2.58±0.01)g的牙鲆(Paralichthys olivaceus)幼鱼,8周后观测牙鲆肝脏和肠道蛋白质消化酶活性和基因表达的影响。结果表明,牙鲆肠道胰蛋白酶的活性不受饲料中大豆异黄酮的显著影响(P>0.05),却随大豆皂甙添加量的升高而显著下降(P<0.05)。肠道糜蛋白酶的活性在饲料中大豆异黄酮添加量为0.1%时取得最大值(37.3±5.9)U/mg,显著高于其它处理组。饲料中添加不同水平的大豆异黄酮对肝脏中胰蛋白酶-1和糜蛋白酶-1基因表达量有显著(P<0.05)的影响,而添加大豆皂甙却对这些基因表达量的影响不显著。由此可见,大豆异黄酮直接作用于基因表达和酶活两个水平,而大豆皂甙只在酶活水平发挥显著的作用。同时,大豆异黄酮对牙鲆饲料利用率影响的双重性依赖于剂量的大小,其中的作用机制有待于进一步研究。  相似文献   

FURTHER OBSERVATION OF THE SPERMATOZOA OF LEFT-EYE FLOUNDER PARALICHTHYS OLIVACEUS BY ELECTRONIC MICROSCOPY     

王宏田  张培军  谢嘉琳  姜明 《中国海洋湖沼学报》2002,20(2)

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牙鲆耐寒相关基因CIRP、HMGB1的克隆及表达特征分析     

胡金伟  尤锋  王倩  王丽娟  翁申达  辛梦娇 《海洋科学》2015,39(1):29-38

为鲆鲽鱼耐低温标记辅助育种研究和应用提供候选基因,并了解耐寒相关基因在低温下的表达图示,作者通过同源克隆以及5?/3?RACE的方法扩增得到了牙鲆(Paralichthys olivaceus)CIRP和HMGB1基因。组织表达谱分析显示,这两个基因在脑、鳃、心脏、肌肉、肝脏、肠等组织均有表达,但HMGB1基因在鳃中表达较弱。低温胁迫后,两基因随着胁迫时间的延长其表达量在肌肉中都出现先上升后下降的趋势:在0~12 h逐渐上升并达到最大值,然后下降恢复至最初水平。但在肝脏和脑组织中的变化趋势却不同,低温胁迫后0~2 h HMGB1基因在脑中的表达量上升至最高,然后下降至最初水平,在肝脏中变化不大。而CIRP基因在低温胁迫后0~2 h在肝脏中表达量处于上升趋势,然后下降至初始水平,在脑中变化不大。由此推测,这两个基因在低温条件下都参与了机体对外界的抵抗,特别是在抵抗外界寒冷的主要组织—肌肉中发挥作用,可能共同参与了机体的免疫反应,对低温胁迫环境下的鱼体进行保护,可以作为鱼类耐低温标记筛选的候选基因。  相似文献   

雌二醇(E2)与塑化剂(DIOP)对褐牙鲆幼鱼组织损伤初步研究     

刘志峰  洪磊  郝振林  雷霁霖 《海洋湖沼通报》2015,(1):96-106

分别采用浓度0.1μmol/L、1μmol/L的雌二醇(E2)和塑化剂(邻苯二甲酸二异辛酯,DIOP)对褐牙鲆幼鱼进行连续5周的暴露实验,取鳃、肝、肾进行常规石蜡切片,HE染色,显微镜观察。实验结果显示,在2种试剂的暴露胁迫下,鱼体组织产生不同程度的损伤,随着试剂浓度的增加、暴露时间的延长而变化明显。研究表明,E2可以导致鳃小片弯曲变细,末端弯曲膨大,鳃小片断裂,细胞坏死、脱落;肝细胞排列紊乱,肝细胞肿胀,出现脂肪变性和空泡化;肾小管形态崩解,肾小管变细,肾小管管腔变大,肾小球结构遭到破坏。DIOP可以促使鳃小叶之间发生组织增生、粘连,鳃小叶末端弯曲膨大,出现非组织性空腔;肝细胞出现轻微的细胞脂肪变性和空泡化;肾小管形态遭到破坏,出现管细胞受损或不可见现象。  相似文献   

In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus     

宿烽  张士璀  李红岩  郭华荣 《中国海洋湖沼学报》2007,25(2):209-214

In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid (IMI) to the gill cell line of flounder (FG) that collected in the gill of Paralichthys olivaceus, was examined by 3 widely used endpoint bioassays: NR (neutral red), MTT (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) and TCP (total cell protein). The result shows that the IMI increased at concentrations ≥0.5 μg/ml. The IC50 value of NR, MTT, and TCP was 41.86, 38.46, and 39.08 μg/ml, respectively. The ultrastructural observation revealed that the mitochondria of the cells exposed to 60 μg/ml IMI for 48 h were severely damaged, swollen or disrupted, while their nuclei and rough endoplasmic reticulum (RER) remained normal. This would suggest that the mitochondria are probably the primary target of IMI.  相似文献   

Genetic analysis of meio-and mito-gynogenetic stocks of Paralichthys olivaceus with microsatellite markers     

WANG Wei  YOU Feng  XU Jianhe  SUN Wei  ZHU Xiangping  GAO Tianxiang  ZHANG Peijun 《海洋学报(英文版)》2008,27(2):149-156

Twenty microsatellite markers （ Pol, Po13, Po33, Po35, Po42, Po48, Po56, Po89, Po91, kop6, kop7, kop8, kop12, kop15, kop18, kop21, kop22, kop23, kop26, Po-str1 ） were used to assess the meiogynogenetic and mitogynogenetic stocks of the lefteyed flounder, Paralichthys olivaceus, which were derived from single pair crossing. Twelve of the 20 loci utilized showed heterozygosity in the female and were mapped in relation to their centromeres in the meiogynogenetic diploid flounder. Microsatellitecentromere map distance, calculated under the assumption of complete interference, ranged from 15. 8 cM for kop22 to 50 cM for Po13, Po56 and Po89. Excluding the kop22, the heterozygosities of the rest of the loci were close to 100% , suggesting the occurrence of near complete interference on the chromosome arms that carried these loci. In the mitogynogenetic diploid flounder, each individual showed exact homozygousity and the segregation profiles did not deviate from the Mendelian 1：1 pattern. The results indicated that there was no lethal gene linked with the loci analyzed. Such high interference accounted for the high recombination rates and large map distances. The Po13 and Po56 loci, Po91 and kopl8 loci, kopl5 and kop21 loci are tightly linked on the same chromosome arm in pairs.  相似文献   

Screening of eye-position related genes with DD-RT-PCR and RDA in the hybrids between Japanese flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus     

陈妍婕  张全启  齐洁  孙业盈  钟其旺  王旭波  王志刚  李朔  李春梅 《中国海洋湖沼学报》2009,27(1)

Flatfish or flounder moves one eye to change body proportion into vertebral asymmetry during metamorphosis, during which some become sinistral while others dextral. However, the mechanism behinds the eye-position has not been well understood. In this research, hybrids between Japanese flounder(♀) and stone flounder (♂) show mixed eye-location in both dextral type and sinistral type, and thus become good samples for studying the eye-migration. mRNAs from pro-metamorphosis sinistral and dextral hybrids larvae...  相似文献   

Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus： karyotype, allozyme and RAPD analyses   总被引：2，自引：0，他引：2  

尤锋  王伟  徐冬冬  朱香萍  倪静  吴志昊  徐永立  王新成  张培军 《中国海洋湖沼学报》2009,27(2):317-323

The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish. Supported by National Natural Science Foundation of China (No. 30571445), National High-Technology Research and Development Program (863 Program, No. 2006AA10A404), and Project from the Ministry of Science and Technology of China (No. 2006DKA30470-017)  相似文献   

牙鲆(Paralichthys olivaceus)消化道形态学及组织学研究   总被引：3，自引：0，他引：3  

刘云  姜国良  郑家声 《中国海洋大学学报(自然科学版)》2001,31(4):548-554

采用大体解剖及光镜技术研究了牙鲆消化道形态学和组织学。牙鲆消化道由口咽腔、食道、胃、小肠、幽门垂和直肠构成。牙鲆口咽腔较大 ,牙锥形 ,为颌齿 ,舌三角形 ,粘膜表面为复层扁平上皮 ;食道粘膜也衬着复层扁平上皮 ,在食道起始处存在少量味蕾和较多的杯状细胞。由食道到胃 ,杯状细胞逐渐减少直至消失。肌肉也由横纹肌变成平滑肌。胃稍膨大 ,弯曲呈 J型 ,粘膜上皮为单层柱状上皮。贲门部、胃体和幽门部均有胃腺分布 ,尤以胃体处最多。小肠短 ,为体长的 1/ 2左右。小肠上皮为具微绒毛的单层柱状上皮 ,幽门垂 4个 ,无括约肌 ,组织结构与小肠相似。直肠粘膜上皮为假复层柱状上皮 ,粘膜上皮中散布着较多的杯状细胞 ,环肌比较发达。牙鲆的消化道组织特点表明 ,其消化过程的特性就是食物停留胃内的时间较短 ,因而牙鲆的进食间隙也较短。  相似文献   

牙鲆鳃淋巴样组织内免疫相关细胞的超微结构   总被引：2，自引：0，他引：2  

刘云  姜国良  姜明  张士璀 《中国海洋大学学报(自然科学版)》2001,31(6):872-876

利用电镜技术观察海水鱼类牙鲆鳃小片的基本结构及其免疫相关细胞的分布和形态。牙鲆的鳃小片主要由扁平上皮细胞和柱细胞构成 ,血窦腔极为发达。鳃小片在功能上可分为两个区 :气体交换区和免疫区。气体交换区位于上半部分 ,血窦内主要分布着红细胞 ;免疫区位于鳃小片基部 ,血窦腔中分布着各种免疫相关细胞 :淋巴细胞、单核细胞、中性粒细胞和酸性粒细胞。此外 ,还观察到了泌氯细胞和粘液细胞。研究结果证明 ,牙鲆的鳃在局部粘膜免疫中具有重要的作用 ,是鱼类免疫体系中的有机组成成分之一  相似文献